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| ID | Components | Size |
| EP101-C01 | High-bind Plate | 1 plate |
| EP101-C02 | Human PD-L1 | 25 μg |
| EP101-C03 | Anti-PD-1 Neutralizing Antibody | 20 μg |
| EP101-C04 | Human PD-1-Biotin | 5 μg |
| EP101-C05 | Streptavidin-HRP | 10 μg |
| EP101-C06 | Coating Buffer | 12 mL |
| EP101-C07 | 10xWashing Buffer | 50 mL |
| EP101-C08 | Blocking Buffer | 50 mL |
| EP101-C09 | Substrate Solution | 12 mL |
| EP101-C10 | Stop Solution | 7 mL |
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
INHIBITION OF PD-1-PD-L1 BINDING BY ANTI-PD-1 NEUTRALIZING ANTIBODY (CATALOG # EP101-C03) MEASURED USING THE PD-1 [BIOTINYLATED] : PD-L1 INHIBITOR SCREENING ELISA ASSAY PAIR (CATALOG # EP-101).
Anti-PD-1 neutralizing antibody was diluted from 10 μg/mL to 0.078 μg/ mL (69.628 nM to 0.544 nM) and loaded onto the plate coated by human PD-L1 in the presence of human PD-1-Biotin. Assay was performed according to the protocol in PD-1[Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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