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Your Position: Home > Antibody > EGF R > HGS-S264

Recombinant Monoclonal Anti-EGFR Antibody, Rabbit (5A6)

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  • Background
    Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules 13. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration. Plays a role in enhancing learning and memory performance (By similarity).
  • Host Species

    Rabbit

  • Clone

    5A6

  • Application

    IHC

  • Property
    1:1000
  • State
    Liquid
  • Positive Control
    Human placentaTissues
  • Clonality
    Monoclonal
  • Synonym
    ERBB, ERBB1
  • Research Field
    Cancer Drug Targets
  • Source
    Rabbit
  • Isotype
    IgG
  • Storage
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. The shelf life is 30 days from the date of opening.
Typical Data
Control Sample
 EGF R CONTROL SAMPLE

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelled with HGS-S264 at 1/1000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Cancer Sample
 EGF R CANCER SAMPLE EGF R CANCER SAMPLE EGF R CANCER SAMPLE

Immunohistochemical analysis of paraffin embedded human cancer tissue labelled with HGS-S264 at 1/1000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $200.00

Price(USD) : $685.00

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Drug Development Status

  • Number of Launched Drugs:33 Details
  • Number of Drugs in Clinical Trials:182 Details
  • Latest Research Phase:Approved

Datasheet & Documentation

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DMF (Drug Master File)

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