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| Assay Type | Sandwich-ELISA |
| Analyte | AAV8 |
| Format | 96T |
| Regulatory Status | RUO |
| Sensitivity | <1.75E+07capsids/mL |
| Standard Curve Range | 5.60E+08 capsids/mL-1.75E+07 capsids/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the detection and quantitative determination of AAV8 capsid titration in AAV gene therapy product preparation processing. |
| Sample volume | 100 uL |
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| AAV08-C01 | Pre-coated Anti-AAV8 Antibody Microplate | 1 plate |
| AAV08-C02 | AAV8 Standard | 1.4E+10 capsids |
| AAV08-C03 | Biotin-Anti-AAV8 Antibody | 15 μg |
| AAV08-C04 | Streptavidin-HRP | 50 μL |
| AAV08-C05 | 10×Washing Buffer | 50 mL |
| AAV08-C06 | 2×Dilution Buffer | 50 mL |
| AAV08-C07 | Substrate Solution | 12 mL |
| AAV08-C08 | Stop Solution | 7 mL |
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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