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| Assay Type | Sandwich-ELISA |
| Analyte | AAV8 |
| Format | 96T |
| Regulatory Status | RUO |
| Sensitivity | <1.94E+08capsids/mL |
| Standard Curve Range | 6.22E+09 capsids/mL-1.94E+08 capsids/mL |
| Assay Time | 2hr 20 min |
| Suitable Sample Type | For the detection and quantitative determination of AAV8 capsid titration in AAV gene therapy product preparation processing. |
| Sample volume | 100 uL |
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| AAV08H-C01 | Pre-coated Anti-AAV8 Antibody Microplate | 1 plate |
| AAV08H-C02 | AAV8 Standard | 1.4E+10 capsids |
| AAV08H-C03 | HRP-Anti-AAV8 Antibody | 15 μg |
| AAV08H-C04 | 10×Washing Buffer | 50 mL |
| AAV08H-C05 | 2×Dilution Buffer | 50 mL |
| AAV08H-C06 | Substrate Solution | 12 mL |
| AAV08H-C07 | Stop Solution | 7 mL |
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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