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| Items | Size （2mg） | Size（5mg X 2） |
| Particle size | 2 μm | 2 μm |
| Physical appearance | Powder mixture | Powder mixture |
| Amount of Coupled Protein | ≈293 pmol (23 μg) Spike S1/mg Beads | ≈293 pmol (23 μg) Spike S1/mg Beads |
| Binding Capacity | >40 μg anti-SARS2-CoV-2 Spike S1 antibody/mg beads; >20 μg ACE2 protein/mg beads | >40 μg anti-SARS2-CoV-2 S1 antibody/mg beads; >20 μg ACE2 protein/mg beads |
| Formulation | PBS, pH7.4, with 10% Trehalose | PBS, pH7.4, with 10% Trehalose |
| Reconstitution | 2 mL sterile deionized water (1 mg beads/mL) | 5 mL sterile deionized water (1 mg beads/mL) |
See Certificate of Analysis (CoA) for detailed instruction.
The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
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Immobilized 25 μg SARS-CoV-2 S1 protein to 1mg Beads, can bind the Anti-SARS-CoV-2 Spike S1 Antibody with an EC50 of 1.003 μg/mL.
Immobilized 25 μg SARS-CoV-2 S1 protein to 1mg Beads, can bind the Human ACE2, Fc Tag (AC2-H5257) with an EC50 of 1.025 μg/mL.
The binding curves between SARS-CoV-2 S1 pre-coupling magnetic beads (Cat. No. MBS-K001) after different freeze-thaw cycles and anti-SARS-CoV-2 S1 antibody. 0.1 mg of Beads (1 mg/ml, 100 μl) was washed three times and the supernatant was removed. 100 μL antibodies of the corresponding concentration (10 μg/ml-0.039 μg/ml) were added. Fluorescent labeled secondary antibody was added for detection (Routinely tested).
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