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This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 20.0 kDa. The protein migrates as 28-38 kDa under reducing (R) condition, and 26-36 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human CD7 Protein, His,Avitag, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Human CD7 Protein, His,Avitag, premium grade (Cat. No. CD7-H82E4) is more than 90% and the molecular weight of this protein is around 52-62 kDa verified by SEC-MALS.
2e5 of Anti-CD7 CAR-293 cells were stained with 100 μL of 3 μg/mL of Biotinylated Human CD7 Protein, His,Avitag, premium grade (Cat. No. CD7-H82E4) and negative control protein respectively, washed and then followed by PE-Streptavidin and analyzed with FACS (QC tested).
Immobilized Anti-CD7 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD7 Protein, His,Avitag, premium grade (Cat. No. CD7-H82E4) with a linear range of 0.5-8 ng/mL (QC tested).
Immobilized Biotinylated Human CD7 Protein, His,Avitag, premium grade (Cat. No. CD7-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-CD7 antibody, Mouse IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).
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