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Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of IL-7 RA and TSLP R, has a calculated MW of 51.7 kDa (IL-7 RA) and 50.3 kDa (TSLP R). Subunit IL-7 RA is fused with a human IgG1 Fc fragment at the C-terminus and subunit TSLP R is fused with a human IgG1 Fc fragment at the C-terminus. The reducing (R) protein migrates as 60-80 kDa due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILR-H5255) is more than 85% and the molecular weight of this protein is around 125-140 kDa verified by SEC-MALS.
Immobilized Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILR-H5255) at 5 μg/mL (100 μL/well) can bind Human TSLP Protein, His Tag, premium grade (Cat. No. TSP-H52Hb) with a linear range of 0.4-3 ng/mL (QC tested).
Serial dilutions of Monoclonal Anti-Human TSLP Antibody, Human IgG2 were added into Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILR-H5255): Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) binding reactions. The half maximal inhibitory concentration (IC50) is 1.09688 μg/mL (Routinely tested).
Loaded Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILR-H5255) on Protein A Biosensor, can bind Human TSLP Protein, His Tag, premium grade (Cat. No. TSP-H52Hb) with an affinity constant of 134 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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