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| ID | Components | Size |
| RAS019-C01 | Pre-coated SARS-CoV-2 Spike RBD Microplate | 1 plate |
| RAS019-C02 | Anti-SARS-CoV-2 Antibody (Control, Monkey IgG) | 100 μL |
| RAS019-C03 | SARS-CoV-2 Antibody Positive Control | 100 μL |
| RAS019-C04 | SARS-CoV-2 Antibody Negative Control | 100 μL |
| RAS019-C05 | HRP-Conjugated Antibody | 100 μL |
| RAS019-C06 | 10xWashing Buffer | 50 mL |
| RAS019-C07 | Dilution Buffer | 50 mL |
| RAS019-C08 | Substrate Solution | 12 mL |
| RAS019-C09 | Stop Solution | 7 mL |
This kit is developed for serologic test for Monkey IgG titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted Secondary antibody HRP-Anti-Goat anti monkey IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Monkey IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Monkey IgG in 1:50 monkey serum. Detection was performed using HRP-Goat anti-Monkey IgG antibody with sensitivity of 98 ng/mL (QC tested).
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