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Your Position: Home > Kits > VEGF165 > EP-137

VEGFA : VEGFR2 [Biotinylated] Inhibitor Screening ELISA Kit

For research use only.

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    Materials Provided
    IDComponentsSize
    EP137-C01High-bind Plate1 plate
    EP137-C02Human VEGFA10 μg
    EP137-C03Biotinylated Human VEGFR220 μg
    EP137-C04Anti-VEGFA Neutralizing Antibody20 μg
    EP137-C05Streptavidin-HRP10 μg
    EP137-C06Coating Buffer12 mL
    EP137-C0710xWashing Buffer 50 mL
    EP137-C08Blocking Buffer50 mL
    EP137-C09Substrate Solution12 mL
    EP137-C10Stop Solution7 mL
  • Product Overview
    VEGFA : VEGFR2 [Biotinylated] Inhibitor Screening ELISA Kit is based on a competition-ELISA method, is used to screening for inhibitors of human VEGFA binding to human VEGFR2. The kit is designed to provide a reliable solution for the screening of antibodies and small molecule inhibitors in the early stages, as well as QC release, to help researchers quickly screen and validate drugs, effectively improving research and development efficiency. It can also be used as a universal detection tool to identify the ability of human VEGFA binding to human VEGFR2.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Typical Data Please refer to DS document for the assay protocol.
     VEGF165 TYPICAL DATA

    INHIBITION OF VEGFA: VEGFR2 [Biotinylated] BINDING BY ANTI-VEGF NEUTRALIZING ANTIBODY
    Serial dilutions of Anti-VEGFA Neutralizing antibody (Catalog # EP137-C04) (1:1 serial dilution, from 10 μg/mL to 0.01953125μg/mL) was added into VEGFA: Biotinylated VEGFR2 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

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