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Your Position: Home > Cell > CD155 > SCRAJ-STT076

Raji/Human CD155 Stable Cell Line

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  1. Genetically modified cell lines best reflect MOA (Mechanism of Action)
  2. Higher activity and larger assay window for robust and reproducible cell-based bioassay
  3. Comprehensive application data to support assay development and validation
  4. Full tracible record, stringent quality control and validated cell passage stability
  5. Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
  6. Global commercial license assistance whenever regulatory filing is required
  • Description
    The Raji/Human CD155 Stable Cell Line was engineered to express full length human CD155 (Uniprot: P15151-1), used to mimic cancer target cells. When co-cultured with human TIGIT Reporter Cell, the TIGIT/CD155 interaction inhibits TCR signaling and NFAT-mediated luminescence. Blocking the TIGIT/CD155 interaction by either anti-TIGIT or anti-CD155 antibodies releases the inhibitory signal and results in TCR activation and NFAT-mediated luminescence.
  • Application

    • Useful for cell-based CD155 binding assay

    • Useful as CD155-expressing target cells in reporter gene assay

     CD155 Assay Principles

  • Growth Properties
    Suspension
  • Selection Marker
    Hygromycin B (20 μg/mL)
  • Complete Growth Medium
    RPMI-1640 + 10% FBS
  • Freeze Medium
    Serum-free cell cryopreservation medium
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Mycoplasma Testing
    Negative
  • Sterility Testing
    Negative
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.

Receptor Assay
 CD155 FACS

Expression analysis of human CD155 on Raji/Human CD155 Stable Cell by FACS.
Raji/Human CD155 Stable Cell Line or negative control cell were stained with PE-labeled anti-Human CD155 antibody.

Application
 CD155 APPLICATION

Blocking activity of anti-human TIGIT antibody.
This Raji/Human CD155 Stable Cell Line was incubated with serial dilutions of antibodies in the presence of reporter cells expressing human TIGIT. The EC50 of anti-human TIGIT antibody was approximately 2.05 μg/mL.

Passage Stability
 CD155 PASSAGE

Passage stability analysis of receptors expression by FACS.
Flow cytometry surface staining of human CD155 on Raji/Human CD155 Stable Cell Line demonstrates consistent mean fluorescent intensity across passage 5-28.

Please contact us if you are interested in related cell pool service.

  • Limited Use&License Disclosure

    BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE FOLLOWING TERMS OF LIMITED USE OF THIS CELL LINE PRODUCT.

    1. If the researcher is not willing to accept the terms of limited use of this cell line product, and the product is unused, ACRO will accept return of the unused product.
    2. Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for profit or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research.
    3. This cell line is neither intended for any animal or human therapeutic purposes nor for any direct human in vivo use . You have no right to share, modify, transfer, distribute, sell, sublicense, or otherwise make the cell line available for use to other researchers, laboratories, research institutions, hospitals, universities, or service organizations.
    4. ACROBIOSYSTEMS MAKES NO WARRANTIES OR REPRESENTATIONS OF ANY KIND, EITHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE SUITABILITY OF THE CELL LINE FOR ANY PARTICULAR USE.
    5. ACROBIOSYSTEMS ACCEPTS NO LIABILITY IN CONNECTION WITH THE HANDLING OR USE OF THE CELL LINE.
    6. Modifications of the cell line, transfer to a third party, or commercial use of the cell line may require a separate license and additional fees. Please contact order@acrobiosystems.com for further details.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $3785.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:2 Details
  • Latest Research Phase:Phase 2 Clinical

Datasheet & Documentation

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