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The kit is developed for quantitative detection of natural and recombinant human IL-6 in serum, plasma and cell culture supernatants.
It is for research use only.
| Assay Type | Sandwich-ELISA |
| Analyte | IL-6 |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
| Reactivity | Human |
| Sensitivity | 2.0 pg/mL |
| Assay Time | 1.75 hr |
| Sample volume | 50 uL |
| Range | 6.25 pg/mL-400 pg/mL |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| NIBSC Code | 89/548 |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IL-6 is less than 2.0 pg/mL.
Serum Sample – 240 samples from healthy volunteers were tested for the presence of human IL-6 concentration in the assay. 187 samples were detectable. No medical histories were available for the donors used in this study.
Cell Culture Supernatant – Human peripheral blood mononuclear cells (PBMCs) were cultured and stimulated with target cells at E:T ratios of 1:10 and 1:5, and then detect the secretion of IL-6 under stimulation with different concentrations of Blinatumomab.
5 types of materials were tested to observe if there were matrix effect (interference). If the concentration of bilirubin (simulated jaundice) is less than 20 mg/dL, the concentration of triglyceride (simulated lipid blood) is less than 3.0 mg/mL, the concentration of hemoglobin (simulated hemolysis) is less than 2% v/v, testing results will not be affected.
High concentrations of human IL-6 serum samples were diluted with 1:2, 1:4, 1:8, 1:16, 1:32 and 1:64 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of IL-6 was 98.43%.
10 replicates of each of 3 samples containing different IL-6 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
3 samples containing different concentrations of IL-6 were tested in the independent assays. Acceptable criteria: CV<15%.
IL-6 was spiked into 3 human serum samples, and then analyzed. The average recovery of IL-6 for serum samples is 94.64%.
| ID | Components | Size |
| CRB001-C01 | Pre-coated Anti-IL-6 Antibody Microplate | 1 plate |
| CRB001-C02 | Human IL-6 Standard | 20 μg×2 |
| CRB001-C03 | Biotin-Anti-IL-6 Antibody Con. Solution | 100 μL |
| CRB001-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CRB001-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CRB001-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CRB001-C07 | 20× Washing Buffer | 50 mL |
| CRB001-C08 | 1× Dilution Buffer | 15 mL×2 |
| CRB001-C09 | Substrate Solution | 12 mL |
| CRB001-C10 | Stop Solution | 6 mL |
Price(USD) : $515.00
Price(USD) :
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