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| Product | Size | Amount |
| ActiveMax® Human CD19 μBeads, premium grade (for cells) | 2.5 mg | 2.5 × 10⁷ beads |
| ActiveMax® Human CD19 μBeads, premium grade (for cells) | 10 mg (2.5 mg × 4) | 1.0 × 10⁸ beads |
See Certificate of Analysis (CoA) for detailed instruction.
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Assay of human CD19 protein on the μBeads surface by Flow cytomtry. The human CD19 conjugated on the μBeads (Cat. No. MBS-C002) surface were fluorescently stained using PE labeled anti-human CD19 antibody and analyzed by flow cytometry (QC tested).
ActiveMax® Human CD19 μBeads, premium grade (for cells) (Cat. No. MBS-C002) can activate CD19-specific CAR-T cells by detecting the secretion of IFN- γ in vitro (Routinely tested).
CD19 CAR-T cells were stimulated with ActiveMax® Human CD19 μBeads (MBS-C002) for 24 h , and cell-free supernatants were harvested for evaluating IFN-γ secretion by ELISA. The results showed that CD19 CAR-T cells released significantly larger amounts of IFN-γ into the supernatants in response to CD19 μBeads . Seed the CD19 μBeads in 96-well plates at a density of 2×10⁴ μBeads/well, and co-culture with CD19 CAR-T cells at an effector-to-target (E:T) ratio of 5:1, 1:1, 1:5 in 96-well tissue culture plate, respectively. CD19 CAR-T cells alone were cultured as the control.
CD19 CAR-T cells were stimulated with ActiveMax® Human CD19 μBeads (MBS-C002) for 24 h , and cell-free supernatants were harvested for evaluating IFN-γ secretion by flow cytomtry. The results showed that CD19 CAR-T cells released significantly larger amounts of IFN-γ into the supernatants in response to CD19 μBeads. Seed the CD19 μBeads in 96-well plates at a density of 2×10⁴ μBeads/well, and co-culture with CD19 CAR-T cells at an effector-to-target (E:T) ratio of 5:1, 1:1, 1:5 in 96-well tissue culture plate, respectively. CD19 CAR-T cells alone were cultured as the control.
CD19 CAR-T cells were stimulated with ActiveMax® Human CD19 μBeads (MBS-C002) for 24h, and cells were harvested for evaluating CD69,CD137 expression by flow cytometry. The results showed that the proportion of CD69 and CD137 in CD19 CAR-T cells was significantly increased after CD19 μBeads stimulation.
Seed the CD19 μBeads in 96-well plates at a density of 2×10⁴ μBeads/well, and co-culture with CD19 CAR-T cells at an effector-to-target (E:T) ratio of 5:1, 1:1, 1:5 in 96-well tissue culture plate, respectively. CD19 CAR-T cells alone were cultured as the control.
CD19 CAR-T cells were stimulated with ActiveMax® Human CD19 μBeads (MBS-C002) for 24h, and cell-free supernatants were harvested for evaluating Granzyme B, Perforin secretion by ELISA. The results showed that CD19 CAR-T cells released significantly larger amounts of Granzyme B, Perforin into the supernatants in response to CD19 μBeads.
Seed the CD19 μBeads in 96-well plates at a density of 2×10⁴ μBeads/well, and co-culture with CD19 CAR-T cells at an effector-to-target (E:T) ratio of 5:1, 1:1, 1:5 in 96-well tissue culture plate, respectively. CD19 CAR-T cells alone were cultured as the control.
After positive selection with the ActiveMax Human CD19 ubeads at 2:4 (beads : cells), the proportion of CAR cells that enriched cells were ≈150-fold higher than that of the initial population - 0.1%.
To simulate difficult enrichment conditions, used CAR cells with low CAR expression. CAR cells in initial stained populations were rare (~ 0.1%). CD19 CAR-T cells were stimulated with ActiveMax® Human CD19 μBeads (MBS-C002) for 24 h. Transfer the tube on a magnet to separate the beads-CAR-T cells from the solution. Carefully pipette off the supernatant to a new tube, this is the unlabeled cell fraction. Wash isolated beads-cells complex (enriched CAR-T cells). Remove the tube from the magnet, resuspend the isolated beads-cells complex and then analyzed with FACS.
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