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Your Position: Home > Kits > OX40 > EP-162

OX40 [Biotinylated] : OX40 Ligand Inhibitor Screening ELISA Kit

For research use only.

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    Materials Provided
    IDComponentsSize
    EP162-C01High-bind Plate1 plate
    EP162-C02Human OX40 Ligand10 μg
    EP162-C03Biotinylated Human OX4010 μg
    EP162-C04Anti-OX40 Neutralizing Antibody10 μg
    EP162-C05Streptavidin-HRP5 μg
    EP162-C06Coating Buffer12 mL
    EP162-C0720xWashing Buffer 50 mL
    EP162-C08Blocking Buffer50 mL
    EP162-C09Substrate Solution12 mL
    EP162-C10Stop Solution7 mL
  • Product Overview
    OX40 [Biotinylated] : OX40 Ligand Inhibitor Screening ELISA Kit is based on a competition-ELISA method, is used to screening for inhibitors of human OX40 binding to human OX40 Ligand. The kit is designed to provide a reliable solution for the screening of antibodies and small molecule inhibitors in the early stages, as well as QC release, to help researchers quickly screen and validate drugs, effectively improving research and development efficiency. It can also be used as a universal detection tool to identify the ability of human OX40 binding to human OX40 Ligand.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    Unopened kit should be stored at 2°C -8°C upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Typical Data Please refer to DS document for the assay protocol.
     OX40 TYPICAL DATA

    INHIBITION OF OX40[Biotinylated]: OX40 Ligand BINDING BY ANTI-OX40 NEUTRALIZING ANTIBODY
    Serial dilutions of Anti-OX40 Neutralizing antibody (Catalog # EP162-C04) (1:1 serial dilution, from 2μg/mL to 0.001μg/mL) was added into OX40[Biotinylated]: OX40 Ligand binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

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