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The kit is developed for quantitative detection of natural and recombinant human TSLP in serum, plasma and cell culture supernatants.
It is for research use only.
| Assay Type | Sandwich-ELISA |
| Analyte | TSLP |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
| Reactivity | Human |
| Sensitivity | <31.25pg/mL |
| Assay Time | 1 hr 45 min |
| Sample volume | 50 uL |
| Range | 31.25 pg/mL-2000 pg/mL |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of TSLP is less than 31.25 pg/mL.
Ten replicates of each of 4 samples containing different TSLP concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Five samples containing different concentrations of TSLP were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant TSLP was spiked into 3 human serum samples, and then analyzed. The average recovery of TSLP for serum samples is 91.5%.
| ID | Components | Size |
| CEA074-C01 | Pre-coated Anti-TSLP Antibody Microplate | 1 plate |
| CEA074-C02 | Human TSLP Standard | 20 ug×2 |
| CEA074-C03 | Biotin-Anti-TSLP Antibody Con. Solution | 100 μL |
| CEA074-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA074-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CEA074-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA074-C07 | 20× Washing Buffer | 50 mL |
| CEA074-C08 | Sample Dilution Buffer | 15 mL×2 |
| CEA074-C09 | Substrate Solution | 12 mL |
| CEA074-C10 | Stop Solution | 6 mL |
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