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| Assay Type | Sandwich-ELISA |
| Analyte | Nuclease |
| Format | 96T(8×12 strips) |
| Reactivity | / |
| Regulatory Status | RUO |
| Sensitivity | ＜25pg/mL |
| Standard Curve Range | 25 pg/mL-800 pg/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the quantitative determination the residue of nuclease in biologicals. |
| Sample volume | 100 uL |
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| RES054-C01 | Pre-coated Anti-Nuclease Antibody Microplate | 1 plate(8×12 strips) |
| RES054-C02 | Salt Active Nuclease Standard | 5 μg |
| RES054-C03 | Biotin-Anti-Nuclease Antibody | 20 μg |
| RES054-C04 | Streptavidin-HRP | 50 μL |
| RES054-C05 | 10×Washing Buffer | 50 mL |
| RES054-C06 | 2×Dilution Buffer | 50 mL |
| RES054-C07 | Substrate Solution | 12 mL |
| RES054-C08 | Stop Solution | 7 mL |
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
Three Salt Active Nuclease with different concentrations were tested to calculate the recovery rate.
resDetect™ Salt Active GENIUS™ Nuclease ELISA Kit (Residue Testing) (Cat. No. RES-A054) can detect the Salt Active Nuclease in different culture supernatant with similar curve and sensitivity.
resDetect™ Salt Active GENIUS™ Nuclease ELISA Kit (Residue Testing) (Cat. No. RES-A054) can detect the Salt Active Nuclease from different culture supernatant and was free from the matrix effects.
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