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Your Position: Home > Kits > Kanamycin > RES-A078

resDetect™ Kanamycin ELISA Kit

For research use only.

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    Product Specifications
    Assay TypeCompetitive-ELISA
    AnalyteKanamycin
    Format96T(8×12 strips)
    Regulatory StatusRUO
    Sensitivity0.5 ng/mL
    Standard Curve Range0.5 ng/mL-40.5 ng/mL
    Assay Time1hr 20 min
    Suitable Sample TypeFor the detection and quantitative determination of kanamycin residues in plasmid DNA raw materials for cell and gene therapy.
    Sample volume50 μL
  • Product Overview
    resDetect™ Kanamycin ELISA Kit is based on the ELISA sandwich method and is used to detect and quantitatively determine Kanamycin residues in plasmid DNA raw materials, proteins for CGT, vaccines, and other biological drug preparations. The kit is calibrated against the USP standard to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of Kanamycin.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Materials Provided
    IDComponentsSize
    RES078-C01Kanamycin Coated Plate1 plate(8×12 strips)
    RES078-C02Kanamycin Standard81ng/mL×1.9mL
    RES078-C03HRP-Anti-Kanamycin Antibody6mL
    RES078-C041×Dilution Buffer50mL
    RES078-C0520xWashing Buffer 50mL
    RES078-C06Substrate Solution12mL
    RES078-C07Stop Solution7mL
  • Assay Principles
    The Kanamycin quantitative detection kit adopts the indirect competitive ELISA method, and the pre-coated conjugated kanamycin antigen on the microstrip competes with the residual kanamycin in the sample to bind the enzyme-labeled anti-kanamycin monoclonal antibody, and then uses a microplate reader to detect the absorbance value by adding TMB substrate, and the absorbance value is negatively correlated with the content of kanamycin in the sample. The kit takes only about one hour and 20 minutes to operate and has a linear range of 0.5 ng/mL to 40.5 ng/mL.
Typical Data Please refer to DS document for the assay protocol.
 Kanamycin TYPICAL DATA

Serial dilutions of kanamycin (from 40.5 ng/mL to 0.5 ng/mL) was added into kanamycin: anti-kanamycin binding reactions. The assay was performed according to the protocol dscribed below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

  • Clinical and Translational Updates

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Price(USD) : $615.00

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