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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 44.4 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Osteoprotegerin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human TNFSF11, Fc Tag (Cat. No. RAL-H5265) at 10 μg/mL (100 μL/well) can bind Human Osteoprotegerin, His Tag (Cat. No. TNB-H5220) with a linear range of 5-156 ng/mL (QC tested).
Immobilized Human Osteoprotegerin, His Tag (Cat. No. TNB-H5220) at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag with a linear range of 0.5-16 ng/mL (Routinely tested).
Loaded Human Osteoprotegerin, His Tag (Cat. No. TNB-H5220) on HIS1K Biosensor, can bind Human TRAIL with an affinity constant of 0.831 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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