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| ID | Components | Size |
| EP168-C01 | High-bind Plate | 1 plate |
| EP168-C02 | Human DR3 | 100 μg |
| EP168-C03 | Human TL1A-SABC | 2.5μg |
| EP168-C04 | Human Anti-TL1A Neutralizing Antibody | 100 μg |
| EP168-C05 | Coating Buffer | 12mL |
| EP168-C06 | 20xWashing Buffer | 50mL |
| EP168-C07 | Blocking Buffer | 50mL |
| EP168-C08 | Substrate Solution | 12mL |
| EP168-C09 | Stop Solution | 7mL |
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Serial dilutions of Human Anti-TL1A Neutralizing antibody (Catalog # EP168-C04) (1:1 serial dilution, from 5 μg/mL to0.0390625 μg/mL) was added into Human DR3 : Biotinylated Human TL1A binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The example data is for reference only.
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