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This protein carries a Flag tag at the N-terminus, and a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 44.7 kDa. The protein migrates as 40-43 kDa and 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
This product is not suitable for cell based experiments due to cytotoxicity of detergent.
Detergent buffer is INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove detergent.
Detergent buffer is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, Buffer A, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human STEAP1 Full Length Protein, Avitag,Flag&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Biotinylated Human STEAP1 Full Length Protein, Avitag,Flag&His Tag (Cat. No. ST1-H82D3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-STEAP1 Antibody with a linear range of 0.002-0.156 μg/mL (QC tested).
Anti-STEAP1 Antibody captured on Protein A Chip can bind Biotinylated Human STEAP1 Full Length Protein, Avitag,Flag&His Tag (Cat. No. ST1-H82D3) with an affinity constant of 12.8 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
Loaded Anti-STEAP1 Antibody on Protein A Biosensor, can bind Biotinylated Human STEAP1 Full Length Protein, Avitag,Flag&His Tag (Cat. No. ST1-H82D3) with an affinity constant of 8.26 nM as determined in BLI assay (in presence of DDM and CHS) (ForteBio Octet R8) (Routinely tested).
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