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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.9 kDa. The protein migrates as 20-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag (Cat. No. CDE-HA2H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) with a linear range of 0.2-8 ng/mL (QC tested).
1e5 of Mouse Anti-CD3 antibody coupled beads (5.5μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag (Cat. No. CDE-HA2H3) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
1e5 of Mouse Anti-CD3 antibody coupled beads (5.5μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag (Cat. No. CDE-HA2H3) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
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