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This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus followed by an Avi tag (Avitag™).
The protein has a calculated MW of 67.9 kDa.
>90% as determined by SDS-PAGE.
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Immobilized Biotinylated Human GLP1R Full Length Protein, Flag Tag&His,Avitag (Cat. No. GLR-H82D3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Glutazumab with a linear range of 0.06-2 ng/mL (QC tested).
Immobilized Biotinylated Human GLP1R Full Length Protein, Flag Tag&His,Avitag (Cat. No. GLR-H82D3) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Dulaglutide with a linear range of 0.5-31 ng/mL (Routinely tested).
Dulaglutide captured on Protein A Chip can bind Biotinylated Human GLP1R Full Length Protein, Flag Tag&His,Avitag (Cat. No. GLR-H82D3) with an affinity constant of 1.91 µM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
Glutazumab captured on Protein A Chip can bind Biotinylated Human GLP1R Full Length Protein, Flag Tag&His,Avitag (Cat. No. GLR-H82D3) with an affinity constant of 30.6 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
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