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Your Position: Home > Protein > MAdCAM-1 > MAM-M82E3

Biotinylated Mouse MAdCAM-1 Protein, His,Avitag™ (MALS verified)

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  • Synonym
    MAdCAM-1,MAdCAM1,Mucosal vascular addressin cell adhesion molecule 1 transcript variant 1
  • Source
    Biotinylated Mouse MAdCAM-1 Protein, His,Avitag(MAM-M82E3) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Ser 364 (Accession # Q61826-1).
    Predicted N-terminus: Gln 22
  • Molecular Characterization
    MAdCAM-1 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 40.4 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
MAdCAM-1 SDS-PAGE

Biotinylated Mouse MAdCAM-1 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
MAdCAM-1 MALS images

The purity of Biotinylated Mouse MAdCAM-1 Protein, His,Avitag (Cat. No. MAM-M82E3) is more than 90% and the molecular weight of this protein is around 55-80 kDa verified by SEC-MALS.

Bioactivity-ELISA
 MAdCAM-1 ELISA

Immobilized Recombinant Mouse Integrin alpha 4 beta 7 Protein at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse MAdCAM-1 Protein, His,Avitag (Cat. No. MAM-M82E3) with a linear range of 1-63 ng/mL (QC tested).

  • Background
    The mucosal addressin cell adhesion molecule-1 (MAdCAM-1) takes a key role in the endothelial adhesion and migration of lymphocytes to sites of inflammation in inflammatory disease. In vitro and in vivo data indicate that blockade of the adhesion molecule mucosal address in cell adhesion molecule (MAdCAM) pathway decreases leukocyte homing to the gut by inhibiting the interaction between MAdCAM and its ligand the α4β7 integrin expressed on lymphocytes.
  • Clinical and Translational Updates

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