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| Assay Type | Sandwich-ELISA |
| Analyte | Trypsin |
| Format | 96T(8×12 strips) |
| Regulatory Status | RUO |
| Sensitivity | ＜0.05ng/mL |
| Standard Curve Range | 0.05ng/mL-3.2ng/mL |
| Assay Time | 1 hr 50 min |
| Suitable Sample Type | For the quantitative determination of Trypsin |
| Sample volume | 50 μL |
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| RES02-C01 | Pre-Coated Anti-Trypsin Antibody Microplate | 1 plate (8×12 strips) |
| RES02-C02 | Trypsin Standard (100 ng/mL) | 200 μL |
| RES02-C03 | Biotin-Anti-Trypsin Antibody | 100 μL |
| RES02-C04 | Streptavidin-HRP | 5 ug |
| RES02-C05 | 1×Dilution Buffer | 50 mL |
| RES02-C06 | 20xWashing Buffer | 50 mL |
| RES02-C07 | Substrate Solution | 12 mL |
| RES02-C08 | Stop Solution | 7 mL |
Detection of Trypsin by ELISA Assay.Immobilized Anti- Trypsin Antibody can bind Trypsin Detection was performed using HRP-Anti- Trypsin Antibody with sensitivity of 0.05 ng/mL (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision , Intra-Assay Precision CV≤15%.
Three samples of known concentration were tested in ten separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
Five samples of known concentration were tested ten times to assess Accuracy , Accuracy recovery rate 80-120%.
We have conducted interference effect test about frequently-used buffers, they have excellent buffer compatibility. For specific buffers, it is recommended that you verify recovery to determine the minimum dilution ratio.
High, medium and low concentrations of Trypsin were added to MDCK(2.8×106 cells/mL), HEK293(4×106 cells/mL) , CHO( 2×106 cells/mL) and Vero( 2.15×106 cells/mL) the recovery rate of Trypsin was used as the specific validation index.
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