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| Traceability | USP (Cat # 1076192) |
| Assay Type | Sandwich-ELISA |
| Analyte | BSA (Bovine serum albumin) |
| Format | 96T(8×12 strips) |
| Regulatory Status | RUO |
| Sensitivity | ＜0.5ng/mL |
| Standard Curve Range | 0.5ng/mL-40.5ng/mL |
| Assay Time | 1.5 hr |
| Suitable Sample Type | For the quantitative determination of BSA (Bovine serum albumin) |
| Sample volume | 50 μL |
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| RES98-C01 | Pre-Coated Anti-BSA Antibody Microplate | 1 plate(8×12 strips) |
| RES98-C02A | BSA Standard (200ng/mL) | 500μL |
| RES98-C02B | BSA Standard 5 (40.5ng/mL) | 500μL |
| RES98-C02C | BSA Standard4 (13.5ng/mL) | 500μL |
| RES98-C02D | BSA Standard 3 (4.5ng/mL) | 500μL |
| RES98-C02E | BSA Standard 2 (1.5ng/mL) | 500μL |
| RES98-C02F | BSA Standard 1 (0.5ng/mL) | 500μL |
| RES98-C02G | BSA Standard 0 (0ng/mL) | 500μL |
| RES98-C03 | HRP-Anti-BSA Antibody | 100μL |
| RES98-C04 | 1×Sample Dilution Buffer | 50mL×2 |
| RES98-C05 | 20×Washing Buffer | 25mL |
| RES98-C06 | Substrate Solution | 12mL |
| RES98-C07 | Stop Solution | 8mL |
Detection of BSA (Bovine Serum Albumin) by ELISA Assay.
Immobilized Anti-BSA Antibody can bind BSA (Bovine Serum Albumin) Detection was performed using HRP-Anti- BSA Antibody with sensitivity of 0.5 ng/mL (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
To assess the linearity of the assay, samples spiked with high concentrations of BSA were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision , Intra-Assay Precision CV≤15%.
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
Five samples of known concentration were tested ten times to assess Accuracy , Accuracy recovery rate 80-120%.
Verify potential matrix effects by adding different levels of specific buffers to the sample buffer. For specific buffers, it is recommended that you verify recovery to determine the optimal dilution ratio.
High, medium and low concentrations of BSA were added to Human serum (v/v 1%), Mouse serum (v/v 2.5%), Human Serum Albumin (1.25mg/mL) and Rabbit serum (v/v 10%), the recovery rate of BSA was used as the specific validation index.
High, medium and low concentrations of BSA were added to MDCK Cell, HEK293 Cell, CHO Cell , T Cell and VERO Cell, the recovery rate of BSA was used as the specific validation index.
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