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Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG Protein, Fc,Avitag&Fc,Avitag is produced by co-expression of IL-2RB and IL-2RA and IL-2RG, has a calculated MW of 53.0 kDa & 77.4 kDa. Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™) and subunit IL-2RA&IL-2RG is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein migrates as 64-70 kDa and 100-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG Protein, Fc,Avitag&Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG Protein, Fc,Avitag&Fc,Avitag (Cat. No. ILG-C82F5) is more than 85% and the molecular weight of this protein is around 190-235 kDa verified by SEC-MALS.
Immobilized Cynomolgus IL-2, His Tag (Cat. No. IL2-C5249) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG Protein, Fc,Avitag&Fc,Avitag (Cat. No. ILG-C82F5) with a linear range of 0.6-10 ng/mL (QC tested).
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