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Specifically recognizes MMAE and does not recognize MMAF.
Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.
M1D12
Rabbit IgG | Rabbit Kappa
Unconjugated
MMAE-BSA.
| Application | Recommended Usage |
| ELISA | 0.06-125 ng/mL |
Protein A purified / Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Immobilized Disitamab Vedotin (RC48) at 0.2 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) (Cat. No. MME-MY2211) with a linear range of 0.06-2 ng/mL (QC tested).
Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) (Cat. No. MME-MY2211) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) (Cat. No. MME-MY2211) at 5 μg/mL, add Disitamab Vedotin (RC48) and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).
Serial dilutions of Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) (Cat. No. MME-MY2211): Disitamab Vedotin (RC48) binding reactions. The half maximal inhibitory concentration (IC50) is 0.006187 μg/mL (Routinely tested).
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