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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 36.0 kDa. The protein migrates as 40-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Alexa Fluor 488-Labeled Mouse CD3 epsilon Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Alexa Fluor 488-Labeled Mouse CD3 epsilon Protein, Fc Tag (Cat. No. CDE-MA256) at 5 μg/mL (100 μL/well) can bind CD3e Monoclonal Antibody with a linear range of 1-63 ng/mL (QC tested).
Immobilized Alexa Fluor 488-Labeled Mouse CD3 epsilon Protein, Fc Tag (Cat. No. CDE-MA256) at 5 μg/mL (100 μL/well) can bind CD3e Monoclonal Antibody with a linear range of 1-63 ng/mL (Routinely tested). Labeling with fluorescent dyes did not affect their activity.
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