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Your Position: Home > Antibody > CD127 > FABm011-02

APC-Labeled Monoclonal Anti-Human CD127 Antibody, Mouse IgG1 (R514) (0.03% Proclin)

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  • Application
    Flow Cytometry (Evaluation of the expression of CD127 on Human cells).
  • Clone
    R514
  • Species
    Mouse
  • Isotype
    Mouse IgG1 | Mouse Kappa
  • Specificity
    This product is a specific antibody specifically reacts with CD127 protein.
  • Reactivity

    Human

  • Immunogen

    Purified Human CD127 Protein.

  • Conjugate

    APC

    Excitation Wavelength: 640 nm

    Emission Wavelength: 661 nm

  • Recommended Dilution
    1:20
  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4, 0.03% Proclin 300, 10% Trehalose, 0.2% BSA.

    Contact us for customized product form or formulation.

  • Storage

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. Store at 2-8 °C for 12 months.
Bioactivity-FACS
 CD127 FACS

Flow cytometric analysis of Human PBMC staining with APC-Labeled Monoclonal Anti-Human CD127 Antibody, Mouse IgG1(R514) (Cat. No. FABm011-02) at 1:20 dilution (5 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. APC signal was used to evaluate the binding activity (QC tested).

  • Background
    Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain. Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor. Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain ( gamma c) to form the functional high affinity IL-7 receptor complex. The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation. Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion. The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R.
  • Clinical and Translational Updates

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Price(USD) : $420.00

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