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Monoclonal Anti-Human IgG3 Fc Antibody, Mouse IgG2a (2B7) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
2B7
Mouse
Mouse IgG2a | Mouse Kappa
Unconjugated
Recombinant Monoclonal
Human.
Recombinant Human IgG3 Fc is expressed from human 293 cells.
Specifically recognizes IgG3 Fc.
| Application | Recommended Usage |
| Western Blot | 10-0.1 μg/mL |
| ELISA | 0.1-125 ng/mL |
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Protein A purified / Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
This product No cross-reactivity in ELISA with
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Monoclonal Anti-Human IgG3 Fc Antibody, Mouse IgG2a (2B7) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Monoclonal Anti-Human IgG3 Fc Antibody, Mouse IgG2a (2B7) (Cat. No. IG3-MY2179) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Immobilized Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG3 (AS35) (Cat. No. SPD-S67) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IgG3 Fc Antibody, Mouse IgG2a (2B7) (Cat. No. IG3-MY2179) with a linear range of 0.1-2 ng/mL (QC tested).
Detection of Monoclonal Anti-Human IgG3 Fc Antibody, Mouse IgG2a (2B7) by Western Blot. Monoclonal Anti-Human IgG3 Fc Antibody, Mouse IgG2a (2B7) at 0.1 μg/mL dilution + Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG3 (AM359b) at 400 ng.
Secondary: (MFC)-Peroxidase AffiniPure GoatAnti-MouseIgG,FcγFragmentSpecificat 1/1500 dilution
Predicted band size: 60 kDa 12% Bis-Tris Protein Gel
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