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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 53.2 kDa.
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Immobilized Alexa Fluor 488-Labeled Human CD5 Protein, His Tag (Cat. No. CD5-HA2H6) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD5 Antibody, Mouse IgG1 (2C8A7) with a linear range of 0.06-2 ng/mL (Routinely tested). Labeling with fluorescent dyes did not affect their activity.
1e5 of Mouse Anti-CD5 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD5 Protein, His Tag (Cat. No. CD5-HA2H6) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
1e5 of Mouse Anti-CD5 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD5 Protein, His Tag (Cat. No. CD5-HA2H6) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
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