Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Kits > TNF-alpha > CEA-C246

ClinMax™ Human TNF-alpha Quick ELISA Kit

For research use only.

Order Now

    Product Overview
    ClinMax™ Human TNF-αlpha Quick ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human TNF-alpha that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.
  • Application

    The kit is developed for quantitative detection of natural and recombinant human TNF-alpha in serum, plasma and cell culture supernatants.

    It is suitable for potency assay of mAbs.

    This Assay has been anchored to NlBSC/WHO standards (Code: 12/154), ClinMax ELISA Kit: NIBSC/WHO Mass Conversion Factor is 1.

    It is for research use only.

  • Workflow
     TNF-alpha Workflow
  • Product Specifications
    Assay TypeSandwich-ELISA
    AnalyteTNF-α
    Format96-wells plate breakable into 12 x 8 wells strips
    ReactivityHuman
    Sensitivity2pg/mL
    Assay Time1.75 hr
    Sample volume50 uL
    Range19.5 pg/mL-1250 pg/mL
    Sample TypeCell Culture Supernatants, Plasma, Serum.
    NIBSC Code12/154

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Typical Data Please refer to DS document for the assay protocol.
 TNF-alpha TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of TNF-α is less than 2.0 pg/mL.

Validation
Sample Values

Human peripheral blood mononuclear cells (PBMCs) obtained were plated at a density of 1 × 10⁶ cells/mL in complete RPMI 1640 medium containing 10% fetal bovine serum, 2 mM L-glutamine, and antibiotic supplements (penicillin 100 U/mL with streptomycin sulfate 100 μg/mL). Following a 5-day incubation period, experimental groups were either maintained as untreated controls or stimulated with 10 μg/mL phytohemagglutinin (PHA). Subsequent analysis involved quantitative measurement of TNF-alpha concentrations in collected culture supernatants using standardized immunoassays.

 TNF-alpha SAMPLE VALUES
Intra-Assay Statistics

Ten replicates of each of 3 samples containing different TNF-α concentrations were tested in one assay. Acceptable criteria: CV<10%.

 TNF-alpha INTRA-ASSAY STATISTICS
Inter-Assay Statistics

3 samples containing different concentrations of TNF-α were tested in inde-pendent assays. Acceptable criteria: CV<15%.

 TNF-alpha INTER-ASSAY STATISTICS
Recovery

Recombinant TNF-α was spiked into 3 human serum samples, and then analyzed. The average recovery of TNF-α for serum samples is 91.3%.

 TNF-alpha RECOVERY
Materials Provided
IDComponentsSize
CEA246-C01Pre-coated Anti-TNF-α Antibody Microplate1 plate(8×12 strips)
CEA246-C02Human TNF-α Standard12.5ng×2
CEA246-C03Biotin-Anti-TNF-α Antibody 20μg
CEA246-C04Biotin-Antibody Dilution Buffer8mL
CEA246-C05Streptavidin-HRP Con. Solution500μL
CEA246-C06Streptavidin-HRP Dilution Buffer15mL
CEA246-C0720× Washing Buffer50mL
CEA246-C08Sample Dilution Buffer15mL ×2
CEA246-C09Substrate Solution12mL
CEA246-C10Stop Solution6mL
  • Clinical and Translational Updates

Comments (0)


ETA of in-stock products: 2 business days

Price(USD) : $565.00

Promotion & Exhibitions



Drug Development Status

  • Number of Launched Drugs:64 Details
  • Number of Drugs in Clinical Trials:71 Details
  • Latest Research Phase:Approved

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message