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The kit is developed for quantitative detection of natural and recombinant human IFN-gamma in serum, plasma and cell culture supernatants.
It is suitable for potency assay of mAbs, CAR-T/NK cell therapy.
This Assay has been anchored to NlBSC/WHO standards (Code: 87/586), ClinMax ELISA Kit: NIBSC/WHO Mass Conversion Factor is 1.
It is for research use only.
| Assay Type | Sandwich-ELISA |
| Analyte | IFN-γ |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
| Reactivity | Human |
| Sensitivity | 20pg/mL |
| Assay Time | 1.75 hr |
| Sample volume | 50 uL |
| Range | 54.7 pg/mL-3500 pg/mL |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| NIBSC Code | 87/586 |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of IFN-γ is less than 20 pg/mL.
Human peripheral blood mononuclear cells (PBMCs) obtained were plated at a density of 1 × 10⁶ cells/mL in complete RPMI 1640 medium containing 10% fetal bovine serum, 2 mM L-glutamine, and antibiotic supplements (penicillin 100 U/mL with streptomycin sulfate 100 μg/mL). Following a 5-day incubation period, experimental groups were either maintained as untreated controls or stimulated with 10 μg/mL phytohemagglutinin (PHA). Subsequent analysis involved quantitative measurement of IFN-γ concentrations in collected culture supernatants using standardized immunoassays.
Ten replicates of each of 3 samples containing different IFN-γ concentrations were tested in one assay. Acceptable criteria: CV＜10%.
3 samples containing different concentrations of IFN-γ were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant IFN-γ was spiked into 3 human serum samples, and then analyzed. The average recovery of IFN-γ for serum samples is 108.9%.
| ID | Components | Size |
| CEA245-C01 | Pre-coated Anti-IFN-γ Antibody Microplate | 1 plate(8×12 strips) |
| CEA245-C02 | Human IFN-γ Standard | 35ng×2 |
| CEA245-C03 | Biotin-Anti-IFN-γ Antibody | 25μg |
| CEA245-C04 | Biotin-Antibody Dilution Buffer | 8mL |
| CEA245-C05 | Streptavidin-HRP Con. Solution | 500μL |
| CEA245-C06 | Streptavidin-HRP Dilution Buffer | 15mL |
| CEA245-C07 | 20× Washing Buffer | 50mL |
| CEA245-C08 | Sample Dilution Buffer | 15mL ×2 |
| CEA245-C09 | Substrate Solution | 12mL |
| CEA245-C10 | Stop Solution | 6mL |
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