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This protein carries an Avi tag (Avitag™) at the N-terminus followed a polyhistidine tag and at the C-terminus by a Flag tag. The protein has a calculated MW of 27.5 kDa. The protein migrates as 25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
>90% as determined by SDS-PAGE.
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, DDM, CHS, pH8.0 with glycerol as protectant.
Contact us for customized product form or formulation.
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human Claudin-4 Full Length Protein, Avitag,His&Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Biotinylated Human Claudin-4 Full Length Protein, Avitag,His&Flag Tag (Cat. No. CL4-H85D3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-Claudin 4 Antibody with a linear range of 0.02-6 ng/mL (QC tested).
Anti-Claudin 4 Antibody captured on Protein A Chip can bind Biotinylated Human Claudin-4 Full Length Protein, Avitag,His&Flag Tag (Cat. No. CL4-H85D3) with an affinity constant of 6.53 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
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