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Recombinant Streptavidin Protein, premium grade

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  • Synonym
    Streptavidin,SA
  • Source
    Recombinant Streptavidin Protein, premium grade(STN-N5114) is expressed from E. coli cells. It contains AA Ala 37 - Ser 163 (Accession # P22629-1).
    Predicted N-terminus: Met
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Molecular Characterization
    Streptavidin Structure

    This protein carries no "tag".

    The protein has a calculated MW of 13.3 kDa. The protein migrates as 14 kDa±3 kDa under reducing (R) condition, and 95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method / rFC method.
  • Host Cell Protein
    <0.5 ng/µg of protein tested by ELISA.
  • Host Cell DNA
    <0.02 ng/μg of protein tested by qPCR.
  • Specific Activity
    ≥15.0 U/mg
  • Sterility
    Negative
  • Mycoplasma
    Negative.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 0.02% NaCl, pH7.3.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Streptavidin SDS-PAGE

Recombinant Streptavidin Protein, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Streptavidin MALS images

The purity of Recombinant Streptavidin Protein, premium grade (Cat. No. STN-N5114) is more than 95% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Streptavidin ELISA

Immobilized Recombinant Streptavidin Protein, premium grade (Cat. No. STN-N5114) can bind Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F4) at 1 μg/mL (100 μL/well) with a linear range of 0.2-13 ng/mL (Routinely tested).

  • Background
    Streptavidin is a 66KDa tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity to biotin. Each unit can bind one biotin. Horseradish peroxidase is metalloenzyme, a 44KDa glycoprotein. When incubate with substrates, it produces a coloured, fluorimetric, or luminescent derivatives, which can be detected and quantified. HRP conjugated Streptavidin is widely used for the detection and quantification of biotinylated proteins.
  • Clinical and Translational Updates

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