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Your Position: Home > Protein > TRPV1 > TR1-H52D3

Human TRPV1 Full Length Protein, Flag,His Tag (Detergent)

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  • Synonym
    VR1, transient receptor potential cation channel subfamily V member 1
  • Source
    Human TRPV1 Full Length Protein, Flag,His Tag(TR1-H52D3) is expressed from human 293 cells (HEK293). It contains AA Lys 2 - Lys 839 (Accession # Q8NER1-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    TRPV1 Structure

    This protein carries flag tag at the N-terminus and polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 98.5 kDa.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    This product is not suitable for cell based experiments due to cytotoxicity of detergent.
    Detergent buffer is INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove detergent.
    Detergent buffer is sold separately and not included in protein, and please contact us if you need the buffer.
    If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, Buffer C, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
Bioactivity-ELISA
 TRPV1 ELISA

Immobilized Human TRPV1 Full Length Protein, Flag,His Tag (Cat. No. TR1-H52D3) at 1 μg/mL (100 μL/well) can bind Anti-TRPV1 antibody with a linear range of 0.3-20 ng/mL (QC tested).

  • Background
    Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. [provided by RefSeq, Jul 2008]
  • Clinical and Translational Updates

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