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Your Position: Home > Protein > CCR2 > CC2-H52D4

Human CCR2 Full Length Protein, Flag,His Tag (Detergent)

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  • Synonym
    C-C chemokine receptor type 2,C-C CKR-2,CC-CKR-2,CC-CKR-2CKR2B,CCR2,CCR-2,CCR2A,CCR2B,CD192 antigen,CD192,chemokine (C-C motif) receptor 2,CKR2,CKR2A,CMKBR2,CMKBR2MGC111760,FLJ78302,MCP-1 receptor,MCP-1-R,MCP-1-RMGC103828,MGC168006,Monocyte chemoattractant protein 1 receptor,monocyte chemotactic protein 1 receptor
  • Source
    Human CCR2 Full Length Protein, Flag,His Tag(CC2-H52D4) is expressed from human 293 cells (HEK293). It contains AA Leu 2 - Ala 374 (Accession # P41597-1).
    Predicted N-terminus: Asp
  • Molecular Characterization
    CCR2 Structure

    This protein carries flag tag at the N-terminus and polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 45.3 kDa.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    This product is not suitable for cell based experiments due to cytotoxicity of DDM.
    DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.
    DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
    If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
*The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.
Bioactivity-ELISA
 CCR2 ELISA

Immobilized Human CCR2 Full Length Protein, Flag,His Tag (Cat. No. CC2-H52D4) at 1 μg/mL (100 μL/well) can bind Anti-CCR2 antibody with a linear range of 0.6-39 ng/mL (QC tested).

Bioactivity-SPR
 CCR2 SPR

Anti-CCR2 antibody captured on Protein A Chip can bind Human CCR2 Full Length Protein, Flag,His Tag (Cat. No. CC2-H52D4) with an affinity constant of 205 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).

  • Background
    The protein encoded by this gene is a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The encoded protein mediates agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This protein can also be a coreceptor with CD4 for HIV-1 infection. This gene is located in the chemokine receptor gene cluster region of chromosome 3.
  • Clinical and Translational Updates

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