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Your Position: Home > Protein > Claudin-9 > CL9-H5283

Human Claudin-9 / CLDN9 Full Length Protein, His,Twin-Strep Tag (Nanodisc)

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  • Synonym
    CLDN9
  • Source
    Human Claudin-9 / CLDN9 Full Length Protein, His,Twin-Strep Tag(CL9-H5283) is expressed from Baculovirus-Insect cells. It contains AA Ala 2 - Val 217 (Accession # O95484-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    Claudin-9 Structure

    This protein carries a twin strep tag at the C-terminus. The protein has a calculated MW of 27.9 kDa. The protein migrates as 22-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution.

    The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein(MSP1D1) upon removal of the detergent.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
*The isotype control of empty/mock nanodisc (Cat. No. APO-H51H3) is sold separately and not included in protein, you can follow this link for product information.
SDS-PAGE
Claudin-9 SDS-PAGE

Human Claudin-9 / CLDN9 Full Length Protein, His,Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 Claudin-9 ELISA

Immobilized Human Claudin-9 / CLDN9 Full Length Protein, His,Twin-Strep Tag (Cat. No. CL9-H5283) at 2 μg/mL (100 μL/well) can bind Anti-Claudin 9 Antibody with a linear range of 0.001-0.313 μg/mL (QC tested).

Bioactivity-SPR
 Claudin-9 SPR

Anti-Claudin 9 Antibody captured on Protein G Chip can bind Human Claudin-9 / CLDN9 Full Length Protein, His,Twin-Strep Tag (Cat. No. CL9-H5283) with an affinity constant of 34.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Claudin-9 belongs to the claudin family. Claudins constitute integral membrane proteins responsible for solute and electrolyte permeability of the tight junction that serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Tight junctions also play a critical role in maintaining cell polarity and signal transductions. Claudin-9 creates charge specific channels in the paracellular space, plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity, is required to preserve sensory cells in the hearing organ because claudin-9-defective tight junctions fail to shield the basolateral side of hair cells from the K+-rich endolymph. Its ion barrier function is essential in the cochlea, but appears to be dispensable in other organs. Is one of the entry cofactors for hepatitis C virus; it enables HCV entry into target cells just as efficiently as CLDN1.
  • Clinical and Translational Updates

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