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The kit is developed for quantitative detection of human IFN alpha 2 in serum, plasma and cell culture supernates.
It is for research use only.
| Assay Type | Sandwich-ELISA |
| Analyte | IFN-α2 |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
| Reactivity | Human |
| Sensitivity | 50pg/mL |
| Assay Time | 1.75 hr |
| Sample volume | 50 uL |
| Range | 312.5 pg/mL-20000 pg/mL |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| NIBSC Code | 94/784 |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of IFN-α2 is less than 50 pg/mL.
Ten replicates of each of 3 samples containing different IFN-γ concentrations were tested in one assay. Acceptable criteria: CV＜10%.
3 samples containing different concentrations of IFN-α2 were tested in inde-pendent assays. Acceptable criteria: CV<15%.
Recombinant IFN-α2 was spiked into 3 human serum samples, and then analyzed. The average recovery of IFN-α2 for serum samples is 103.6%.
| ID | Components | Size |
| CEA240-C01 | Pre-coated Anti-IFN-α2 Antibody Microplate | 1 plate(8×12 strips) |
| CEA240-C02 | Human IFN-α2 Standard | 200ng×2 |
| CEA240-C03 | Biotin-Anti-IFN-α2 Antibody Con. Solution | 100μL |
| CEA240-C04 | Biotin-Antibody Dilution Buffer | 8mL |
| CEA240-C05 | Streptavidin-HRP Con. Solution | 500μL |
| CEA240-C06 | Streptavidin-HRP Dilution Buffer | 15mL |
| CEA240-C07 | 20× Washing Buffer | 50mL |
| CEA240-C08 | Sample Dilution Buffer | 15mL ×2 |
| CEA240-C09 | Substrate Solution | 12mL |
| CEA240-C10 | Stop Solution | 6mL |
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