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Your Position: Home > Protein > IFN-beta > IFB-H5254

Human IFN-beta Protein, Fc Tag (MALS verified)

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  • Synonym
    IFNB1,Interferon beta,IFN-beta,IFB,IFNB
  • Source
    Human IFN-beta Protein, Fc Tag(IFB-H5254) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Asn 187 (Accession # P01574).
    Predicted N-terminus: Met 22
  • Molecular Characterization
    IFN-beta Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 46.5 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IFN-beta SDS-PAGE

Human IFN-beta Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IFN-beta MALS images

The purity of Human IFN-beta Protein, Fc Tag (Cat. No. IFB-H5254) is more than 85% and the molecular weight of this protein is around 95-120 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IFN-beta ELISA

Immobilized Human IFNAR2, His Tag (Cat. No. IF2-H5224) at 5 μg/mL (100 μL/well) can bind Human IFN-beta Protein, Fc Tag (Cat. No. IFB-H5254) with a linear range of 0.03-2 ng/mL (QC tested).

  • Background
    Interferon beta(IFN beta) is type I interferon cytokine that plays a key role in the innate immune response to infection, developing tumors and other inflammatory stimuli. Signals via binding to high-affinity (IFNAR2) and low-affinity (IFNAR1) heterodimeric receptor, activating the canonical Jak-STAT signaling pathway resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response, such as antiviral proteins, regulators of cell proliferation and differentiation, and immunoregulatory proteins. IFNB1 is more potent than interferon-alpha (IFN-alpha) in inducing the apoptotic and antiproliferative pathways required for control of tumor cell growth (By similarity)
  • Clinical and Translational Updates

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Price(USD) : $350.00

Price(USD) : $2310.00

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  • Number of Drugs in Clinical Trials:6 Details
  • Latest Research Phase:Phase 3 Clinical

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