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Your Position: Home > Protein > FCMR > FCR-H82E3

Biotinylated Human FCMR Protein, His,Avitag™ (MALS verified)

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  • Synonym
    FAIM3, FcmuR, TOSO
  • Source
    Biotinylated Human FCMR Protein, His,Avitag(FCR-H82E3) is expressed from human 293 cells (HEK293). It contains AA Arg 18 - Gly 251 (Accession # O60667-1).
    Predicted N-terminus: Arg 18
  • Molecular Characterization
    FCMR Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 30.0 kDa. The protein migrates as 35-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FCMR SDS-PAGE

Biotinylated Human FCMR Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
FCMR MALS images

The purity of Biotinylated Human FCMR Protein, His,Avitag (Cat. No. FCR-H82E3) is more than 90% and the molecular weight of this protein is around 35-55 kDa verified by SEC-MALS.

Bioactivity-ELISA
 FCMR ELISA

Immobilized Biotinylated Human FCMR Protein, His,Avitag (Cat. No. FCR-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Siglec-7&J chain Pentamer, IgM Fc Tag&His Tag (Cat. No. SI7-H5259) with a linear range of 2-78 ng/mL (QC tested).

  • Background
    Fc receptors specifically bind to the Fc region of immunoglobulins (Igs) to mediate the unique functions of each Ig class. FAIM3 encodes an Fc receptor for IgM (see MIM 147020) (Kubagawa et al., 2009 [PubMed 19858324]; Shima et al., 2010 [PubMed 20042454]).[supplied by OMIM, Jul 2010]
  • Clinical and Translational Updates

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