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| Assay Type | Sandwich-ELISA |
| Analyte | IL-4 |
| Format | 96T(8×12 strips) |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | <0.391pg/mL |
| Standard Curve Range | 0.391 pg/mL-25 pg/mL |
| Assay Time | 2 hr 50 min |
| Suitable Sample Type | For the quantitative determination of human IL-4 in Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 100 uL |
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| CRS004-C01 | Pre-coated Anti-IL-4 Antibody Microplate | 1 plate(8×12 strips) |
| CRS004-C02 | Human IL-4 Standard | 20 μg |
| CRS004-C03 | Biotin-Anti-IL-4 Antibody | 50 μL |
| CRS004-C04 | Streptavidin-HRP | 50 μL |
| CRS004-C05 | 10xWashing Buffer | 50 mL |
| CRS004-C06 | 2xDilution Buffer | 50 mL |
| CRS004-C07 | Substrate Solution | 12 mL |
| CRS004-C08 | Stop Solution | 7 mL |
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
To assess the linearity of the assay, samples spiked with high concentrations of human IL-4 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV＜10%.
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<10%.
Three parts of blank serum were added with different concentrations of human IL-4, and the serum without human IL-4 was used as background to calculate the recovery rate. The range of the recovery rate is 80.9-97.7%, and the average recovery is 86.3%.
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