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| ID | Components | Size |
| EP132-C01 | High-bind Plate | 1 plate |
| EP132-C02 | Human IL-4 R alpha | 30 μg |
| EP132-C03 | Human IL-4 | 20 μg |
| EP132-C04 | Human IL-4-Biotin | 10 μg |
| EP132-C05 | Streptavidin-HRP | 10 μg |
| EP132-C06 | Coating Buffer | 12 mL |
| EP132-C07 | 10xWashing Buffer | 50 mL |
| EP132-C08 | Blocking Buffer | 50 mL |
| EP132-C09 | Substrate Solution | 12 mL |
| EP132-C10 | Stop Solution | 7 mL |
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
INHIBITION OF IL-4 [BIOTINYLATED]: IL-4 R ALPHA BINDING BY Human IL-4
Serial dilutions of Human IL-4(Catalog # EP132-C03) (1:1 serial dilution, from 5 μg/mL to 0.01 μg/mL (295.86-0.58 nM)) was added into IL-4 R alpha: IL-4-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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