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This protein carries no "tag".
The protein has a calculated MW of 58.9 kDa. The protein migrates as 76 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IL-12 Protein, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human IL-12 Protein, premium grade (Cat. No. IL2-H5210) is more than 90% and the molecular weight of this protein is around 60-80 kDa verified by SEC-MALS.
Human IL-12 Protein, premium grade (Cat. No. IL2-H5210) stimulates secretion of IFN-γ by NK-92 human natural killer lymphoma cells. The specific activity of Human IL-12 Protein, premium grade is > 1.00ⅹ10^7 IU/mg, which is calibrated against human IL-12 WHO International Standard (NIBSC code: 95/544) (QC tested).
Immobilized Human IL-12 Protein, premium grade (Cat. No. IL2-H5210) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-12 R beta 1, Fc,Avitag (Cat. No. ILB-H82F7) with a linear range of 0.002-0.156 μg/mL (QC tested).
Immobilized Human IL-12 Protein, premium grade (Cat. No. IL2-H5210) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL23A&IL12B P40 domain Antibody, Human IgG1 with a linear range of 0.05-3 ng/mL (Routinely tested).
Loaded Biotinylated Human IL-12 R beta 1, Fc,Avitag (Cat. No. ILB-H82F7) on SA Biosensor, can bind Human IL-12 Protein, premium grade (Cat. No. IL2-H5210) with an affinity constant of 2.6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG IL-12.
Price(USD) : $310.00
Price(USD) : $495.00
Price(USD) : $3605.00
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