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Your Position: Home > Kits > CD47 > EP-138

CD47 [Biotinylated] : SIRP alpha Inhibitor Screening ELISA Kit

For research use only.

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    Materials Provided
    IDComponentsSize
    EP138-C01High-bind Plate1plate
    EP138-C02Human SIRP alpha15μg
    EP138-C03Biotinylated Human CD4710μg
    EP138-C04Anti-CD47 Neutralizing Antibody20μg
    EP138-C05Streptavidin-HRP10μg
    EP138-C06Coating Buffer12mL
    EP138-C0710xWashing Buffer 50mL
    EP138-C08Blocking Buffer50mL
    EP138-C09Substrate Solution12mL
    EP138-C10Stop Solution12mL
  • Product Overview
    CD47 [Biotinylated] : SIRP alpha Inhibitor Screening ELISA Kit is based on a competition-ELISA method, is used to screening for inhibitors of human CD47 binding to human SIRP alpha. The kit is designed to provide a reliable solution for the screening of antibodies and small molecule inhibitors in the early stages, as well as QC release, to help researchers quickly screen and validate drugs, effectively improving research and development efficiency. It can also be used as a universal detection tool to identify the ability of human CD47 binding to human SIRP alpha.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Typical Data Please refer to DS document for the assay protocol.
     CD47 TYPICAL DATA

    INHIBITION OF CD47[Biotinylated]: SIRP alpha BINDING BY ANTI-CD47 NEUTRALIZING ANTIBODY
    Serial dilutions of Anti-CD47 Neutralizing antibody (Catalog # EP138-C04) (1:1 serial dilution, from 10 μg/mL to 0.01μg/mL) was added into Biotinylated CD47: SIRP alpha binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

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    • Latest Research Phase:Phase 3 Clinical

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