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| Assay Type | Indirect-ELISA |
| Analyte | Anti-CD28 Antibody |
| Format | 96T(8×12 strips) |
| Reactivity | / |
| Regulatory Status | RUO |
| Sensitivity | ≤0.078ng/mL |
| Standard Curve Range | 0.078 ng/mL-2.5 ng/mL |
| Assay Time | 2 hr 20 min |
| Suitable Sample Type | For the detection of anti-CD28 antibody in Bioprocess manufacturing applications |
| Sample volume | 100 uL |
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| CRS014-C01 | Pre-coated Human CD28 Microplate | 1 plate(8×12 strips) |
| CRS014-C02 | Anti-CD28 Antibody Standard | 20 μg |
| CRS014-C03 | HRP-Goat anti-Mouse IgG | 10 μg |
| CRS014-C04 | 10xWashing Buffer | 50 mL |
| CRS014-C05 | 2xDilution Buffer | 50 mL |
| CRS014-C06 | Substrate Solution | 12 mL |
| CRS014-C07 | Stop Solution | 7 mL |
Detection of Monoclonal Anti-CD28 Antibody titer by indirect-ELISA Assay.
Immobilized Human CD28 can bind Anti-CD28 Antibody. Detection was performed using HRP-Goat anti-Mouse IgG with sensitivity of 0.16 ng/mL (QC tested).
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