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This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 31.0 kDa. The protein migrates as 33-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Cynomolgus / Rhesus macaque TIGIT Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Cynomolgus / Rhesus macaque TIGIT Protein, His,Avitag (Cat. No. TIT-C82E3) is more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.
Immobilized Human CD155, Fc Tag (Cat. No. CD5-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque TIGIT Protein, His,Avitag (Cat. No. TIT-C82E3) with a linear range of 4-125 ng/mL (QC tested).
Immobilized Anti-TIGIT Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque TIGIT Protein, His,Avitag (Cat. No. TIT-C82E3) with a linear range of 0.06-2 ng/mL (Routinely tested).
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