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Your Position: Home > Antibody > 218 linker > 218-AHFMY3

APC-Labeled Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) (0.03% Proclin)

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  • Source
    APC-Labeled Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) is a monoclonal antibody recombinantly expressed from HEK293 cells, which provides higher batch consistency and long term security of supply.
  • Application
    Flow Cytometry (Evaluation of CAR and SCFV containing 218linker on Human cells).
  • Clone
    P3C09
  • Species
    Rabbit
  • Isotype
    Rabbit IgG | Rabbit Kappa
  • Specificity
    This product is a specific antibody specifically reacts with CAR or scFv containing the 218 linker sequence.
  • Reactivity

    Human

  • Immunogen

    Purified 218 linker protein.

  • Conjugate

    APC

    Excitation Wavelength: 640 nm

    Emission Wavelength: 661 nm

  • Recommended Dilution
    1:50
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 0.2% BSA, 0.03% Proclin300 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months after reconstitution.
    3. 2-8 °C for 12 months after reconstitution.
Bioactivity-FACS
 218 linker FACS

Flow cytometric analysis of Anti-BCMA (C11D5.3) CAR 293 cells staining with APC-Labeled Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) (Cat. No. 218-AHFMY3) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL). APC signal was used to evaluate the binding activity (QC tested).

  • Background
    The whitlow/218 linker, with the sequence GSTSGSGKPGSGEGSTKG, is a commonly used synthetic peptide linker. Whitlow/218 linker often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The scFv containing the 218 linker showed reduced aggregation and was found to be more stable to proteolysis in vitro.
  • Clinical and Translational Updates

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Price(USD) : $245.00

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