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Human
Purified 218 linker protein.
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 0.2% BSA, 0.03% Proclin300 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Flow cytometric analysis of Anti-BCMA (C11D5.3) CAR 293 cells staining with PE-Labeled Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) (Cat. No. 218-PCFMY3) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL). PE signal was used to evaluate the binding activity (QC tested).
Non-specificity of PE-Labeled Monoclonal Anti-Whitlow 218 Linker Antibody binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with APC-Labeled Monoclonal Anti-Human CD3 Antibody and PE-Labeled Monoclonal Anti-Whitlow 218 Linker Antibody, Rabbit IgG (P3C09) (Cat. No. 218-PCFMY3) (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both APC and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
Flow cytometric analysis of Anti-BCMA (C11D5.3) CAR 293 cells staining with PE-Labeled Monoclonal Anti-Whitlow 218 Linker Antibody, Rabbit IgG (P3C09) (Cat. No. 218-PCFMY3) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) and Competitor C. PE signal was used to evaluate the binding activity. The biological activity level of 218-PCFMY3 is superior to Competitor C (Routinely tested).
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